Composite

Part:BBa_K4165072:Design

Designed by: Hossam Hatem   Group: iGEM22_CU_Egypt   (2022-09-30)


T7 promoter-GST-DocS-T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 45
    Illegal SapI.rc site found at 189


Design Notes

This part is optimized to be expressed in E. coli.


Source

this sequence is a modified version of DocS part from NUDT 2020 team (iGEM registry).

References